Characterization of Virus&VLPs
Viruses, though parasitic, are highly biocompatible, stable, and easy to produce—making them ideal tools in gene therapy, vaccine development, and biomedical research.
The Nanocoulter™ Single-Particle Analyzer enables accurate detection of viral particles, providing key metrics including titer, size distribution, zeta potential, and aggregation state—all in a single test.Compatible with lentivirus, adenovirus, AAV, and retrovirus, Nanocoulter™ offers:
- 1 nm resolution
- Multiparameter readout (size, concentration, charge)
- Fast, reproducible results across R&D and manufacturing
Accelerate your viral vector development with real-time, high-resolution data at every stage—from research to QC.
Batch-to-Batch Quality Control in Adenovirus Production
Cell culture conditions, including medium composition, temperature, and pH, influence adenovirus production efficiency. NanoCoulter™ enables real-time monitoring of virus concentration, size distribution, and zeta potential, facilitating rapid batch assessment and process optimization.
Quantifying Lentivirus Purification Efficiency with Single-Particle Precision
Lentivirus purification involves multiple steps such as centrifugation, filtration, ion exchange, and ultrafiltration, each potentially leading to particle loss. The data below, obtained using Nanocoulter™, clearly reveals changes in particle size and concentration before and after purification—providing a powerful tool to evaluate purification efficiency and support process development.
Optimizing Lentivirus Harvest Timing with Real-Time Monitoring
Lentiviral particles are typically harvested from the culture supernatant after a defined incubation period, with the option to continue harvesting through feed strategies. Maximizing viral yield requires accurate, real-time monitoring to guide process development.
Nanocoulter™ enables continuous tracking of viral particle production over time, helping identify the optimal harvest window. In the study below, results show that 50 hours post-inoculation yields the highest viral concentration—offering a data-driven basis for harvest optimization.
Aggregation Analysis of Poxvirus
The virus‘s storage conditions impact aggregation, with increased aggregates reducing infectivity. NanoCoulter™, with its high particle size resolution, is one of the best methods, aside from TEM, for quickly and accurately assessing aggregation. viral particles in Condition 2 display significantly greater dispersion than those in Condition 1.
Quantification of AAV Particle Concentration
AAV Aggregation Analysis and Inter-batch QC
