NanoCoulterTM Vertex MAX builds on the foundation of Vertex with an expanded particle size detection range, enabling simultaneous measurement of size, concentration, and zeta potential.
Unparalleled Detection Performance
Electron Microscopy-Comparable Particle Size Determination
NanoCoulter™ measures both the particle size and size distribution of polydisperse samples, delivering EM-consistent results with exceptional accuracy and precision.
Measure Concentration with High Accuracy and Repeatability
Samples were serially diluted, and the concentration detection data demonstrated a strong linear correlation with the theoretical concentration (R² > 0.999). Across 10 repeated measurements, the coefficient of variation (CV) was less than 5%.
Single-Particle Zeta Potential Measurement
Zeta potential is an important indicator of the stability of dispersion systems. Under a constant electric field, the electrophoretic velocity of particles is directly proportional to the absolute value of their zeta potential. NanoCoulter™ measures the time it takes for particles to pass through a nanopore to obtain Zeta potential data, making it the only technology platform capable of simultaneously measuring single-particle size, Zeta potential, and concentration.
Related Cases
MISEV Recommends RPS for EV Characterization
“RPS measurements do have very high concordance with TEM data.”
—MISEV 2023
RPS technology, a non-optical method, well suited for orthogonal validating against TEM and flow cytometry. NanoCoulter™ offers accurate size distribution analysis with a defined LOD (50nm) and a wide concentration range (5×10⁷-2×10¹¹ particles/mL), making it indispensable for EV research.”
Investigation of EV Isolation Methods
Exosomes have complex origins and often require multiple isolation steps to obtain relatively pure exosomes. Different isolation methods can significant impact on the particle size and concentration of exosomes. The NanoCoulter™ enables rapid and accurate assessment of the advantages and disadvantages of different purification methods.
Quantification of Adeno-Associated Virus (AAV) Particle Concentration
In addition to infectious titer, parameters such as physical titer (total particle concentration), particle size and distribution, purity, particle integrity, and aggregation status are also essential critical quality attributes for AAV gene therapy drug products. The NanoCoulter™ overcomes industry barriers, achieving single-particle nanoscale characterization for particles below 20 nm for the first time. It is particularly well-suited for high-sensitivity particle size analysis and high-precis
Three typical patterns of particle size distribution were observed for AAV samples:
Sample #1: A relatively broad normal distribution with evidence of particle aggregation.
Sample #2: Two distinct normal distribution peaks, with large-particle impurities potentially indicating Oligomer or residues from upstream processing.
Sample #3: A relatively narrow normal distribution, consistent with the typical size distribution of AAV.
The NanoCoulter™ enables precise quantification of total AAV part
Liposome Stability Study
The stability of liposomal drugs directly affects their storage and in vivo delivery performance. The NanoCoulter™ enables precise evaluation of stability differences among various liposomes. The figure below shows the concentration changes of two liposome samples after different durations of simulated agitation, indicating that Sample 2 exhibits superior stability.
LNP Particle Size Distribution and Zeta Potential Analysis
Particle size and size distribution are among the key Critical Quality Attributes (CQAs) parameters for LNPs. LNPs prepared by different methods may exhibit different size distributions, which are often overlooked by DLS measurements. The
NanoCoulter™ can resolve the size distribution of LNP particle populations while providing concentration and component proportions within user-defined size ranges, along with the correlation between Zeta potential and particle size for each individual nanopar
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