NanoCoulterTM Vertex PRO builds on the foundation of Vertex with an expanded particle size detection range, enabling simultaneous measurement of size, concentration, and zeta potential.
Unparalleled Detection Performance
Particle size measurement precision comparable to electron microscopy
The NanoCoulterTM measures the average particle size and size distribution of polystyrene standard microspheres and other polydisperse samples,producing results that are fully consistent with SEM data, offering exceptionally high accuracy and precision.
Measure concentration with high accuracy and repeatability
Samples were diluted in a gradient, and the concentration detection data showed a good linear relationship, consistent with the theoretical concentration（R2 > 0.99）. With 10 repeated tests, the coefficient of variation (CV) was less than 5%.
Single-Particle Zeta Potential Measurement
Zeta potential is an important indicator of the stability of dispersion systems. Under a constant electric field, the speed at which particles move is positively correlated with the absolute value of the Zeta potential. NanoCoulterTM measures the time it takes for particles to pass through a nanopore to obtain Zeta potential data, making it the only technology platform capable of simultaneously measuring single-particle size, Zeta potential, and concentration.
Related Cases
MISEV Recommends RPS for EV Characterization
“RPS measurements do have very high concordance with TEM data.”
—MISEV 2023
RPS technology, a non-optical method, is ideal for orthogonal validating TEM and flow cytometry. NanoCoulter™ offers accurate size distribution analysis with a wide detection range (50-800 nm) and concentrations (5×10⁷-2×10¹¹ particles/mL), making it indispensable for EV research.”
Investigation of EV Isolation Methods
Batch-to-Batch Quality Control for AAV Products
Beyond infectious titer, critical quality attributes of AAV gene therapy products also include physical titer (total particle concentration), particle size and distribution, purity, integrity, and aggregation state.
NanoCoulter™ breaks industry barriers by enabling single-particle characterization below 20 nm for the first time—offering high-sensitivity size analysis and high-precision concentration quantification for small viral particles like AAV.
NanoCoulter™ reveals three typical size distribution patterns in AAV samples:
Sample #1: A broad normal distribution with signs of particle aggregation.
Sample #2: Two distinct peaks, suggesting the presence of larger impurities—possibly dimers or upstream process residues.
Sample #3: A narrow, well-defined distribution consistent with typical AAV particle size.
In addition to high-resolution size profiling, NanoCoulter™ enables highly accurate quantification of total AAV particle concentration, achieving a linear correlation coefficient (R²) of up to 0.999.
Stability Study of Liposomal Drug
NanoCoulter™ precisely evaluates Liposome stability which varies and impacts the drug usage. The concentration changes of two liposome samples after vortex reveals the better stability of Sample 2
Particle Size Composition & Zeta Potential Analysis of LNPs
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