Characterization of Liposomes/LNPs
Liposomes are among the most extensively studied drug delivery vehicles, prized for their biocompatibility and biodegradability.
The Nanocoulter™ platform enables high-resolution, single-particle analysis of liposomes and lipid nanoparticles (LNPs), delivering precise measurements of particle size, concentration, and zeta potential.
This data supports process monitoring, size control, and quality assurance, while also facilitating in-depth studies on aggregation, stability, and storage conditions.
Nanocoulter™ is an essential tool for accelerating the development and production of liposomal drug formulations.
Nanocoulter™ models for Lipid-based nanoparticles research
Sizing with TEM Accuracy. No TEM Required.
TEM data from different batches of liposome samples visually demonstrate the high precision, resolution, and accuracy of NanoCoulter™ detection.
Stability Study of Lipid-based Drugs
NanoCoulter™ precisely evaluates lipid-based drug delivery systems stability which varies and impacts the drug usage. Minimal changes in size and concentration after vortexing over varying durations reveal the superior stability of Sample 2.
Optimizing Liposome Manufacturing Methods
Different preparation methods can result in significant variations in liposome /LNPs particle size, making it essential to have a high-precision characterization method to guide the selection of the optimal production process. NanoCoulter can identify the most suitable preparation method. The figure below shows the particle size distributions of liposomes produced by four different methods. Method three demonstrates a uniform particle size distribution and higher concentration, outperforming the other three methods.
Particle Size Composition & Zeta Potential Analysis of LNPs
Particle size and distribution are CQA for LNP drugs, varying with preparation methods and these differences are often misrepresented by DLS. NanoCoulter™ accurately analyzes size distribution, concentration, and percentage of custom-gated size fractions, while precisely correlating individual particle size with zeta potential across various LNP formulations.
